Hillenmeyer ME~Giaever G, 2008

Pubmed ID 18420932
Title The chemical genomic portrait of yeast: uncovering a phenotype for all genes.
Authors Maureen E Hillenmeyer, Eula Fung, Jan Wildenhain, Sarah E Pierce, Shawn Hoon, William Lee, Michael Proctor, Robert P St Onge, Mike Tyers, Daphne Koller, Russ B Altman, Ronald W Davis, Corey Nislow, Guri Giaever
Abstract Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.
Citation Science 2008; 320:362-5

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,3-Diallylurea [20 mM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,3-Diallylurea [10 mM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,4-Dimethylendothall [100 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,2:7,8-diepoxyoctane [0.25%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,8-Nonadiene [0.49%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 1,8-Nonadiene [0.99%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 2-CYANOETHYL ACRYLATE [360 mM], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [23ºC], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [23ºC], time [o/n + 15 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [25ºC], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [25ºC], time [o/n + 15 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [37ºC], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) temperature [37ºC], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 4-nitroquinoline N-oxide [0.0313 uM], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [9.6 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [19.1 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [19.2 uM], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [19.2 uM], time [o/n + 15 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [38.46 uM], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [38.46 uM], time [o/n + 15 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [38.46 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 5-fluorouracil [250 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) acetic acid [50 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) aclacinomycin A [32.25 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) acriflavinium chloride [250 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) time [o/n + 5 gen] SC - Ade hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) Adenophostin A [500 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) MLS002701581 [500 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) aclacinomycin T hydrochloride [250 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) alizarin [66 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) diallyl disulfide [0.01%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) diallyl disulfide [0.016%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) diallyl disulfide [0.06%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) Diallyl sulfide [0.12%], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) alverine citrate [500 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) amphotericin B [0.31 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) amsacrine [500 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) angelicin [62.5 uM], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) angelicin irradiated YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) time [o/n + 5 gen] SC - Arg hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) aureobasidin A [0.02 ug/ml], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [75 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [100 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) carmustine [62.5 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) BCS [100 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) BCS [200 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) benomyl [10 ug/ml], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) benomyl [10 ug/ml], time [o/n + 5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) benzaldehyde [0.003%], time [5 gen] YPD hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (pooled culture) Berberine chloride (TN) [125 uM], time [o/n + 20 gen] YPD hom 4,742 Quantitative

Curation history

Data

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Tested strains

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