Hillenmeyer ME~Giaever G, 2008

Pubmed ID 18420932
Title The chemical genomic portrait of yeast: uncovering a phenotype for all genes.
Authors Maureen E Hillenmeyer, Eula Fung, Jan Wildenhain, Sarah E Pierce, Shawn Hoon, William Lee, Michael Proctor, Robert P St Onge, Mike Tyers, Daphne Koller, Russ B Altman, Ronald W Davis, Corey Nislow, Guri Giaever
Abstract Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.
Citation Science 2008; 320:362-5
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Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 1,3-diallylurea [20 mM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 1,3-diallylurea [10 mM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], 1,4-dimethylendothall [100 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], 1,7-octadiene [0.25%] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 1,8-nonadiene [0.49%], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 1,8-nonadiene [0.99%], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [5 generations], 2-cyanoethyl acrylate [360 mM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) temperature [23ºC], time [overnight + 5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 15 generations], temperature [23ºC] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], temperature [25ºC] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 15 generations], temperature [25ºC] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], temperature [37ºC] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], temperature [37ºC] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 4-nitroquinoline N-oxide [0.0313 uM], time [5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], 5-fluoro-uracil [9.6 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 5-fluoro-uracil [19.1 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], 5-fluoro-uracil [19.2 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 15 generations], 5-fluoro-uracil [19.2 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], 5-fluoro-uracil [38.46 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 5-fluoro-uracil [38.46 uM], time [overnight + 15 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], 5-fluoro-uracil [38.46 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) 5-fluoro-uracil [250 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], acetic acid [50 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], aclarubicin [32.25 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) acriflavinium hydrochloride [250 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], minimal complete -Ade [standard] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], adenophostin A [500 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], AG 957 [500 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], aklavin hydrochloride [250 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], alizarin [66 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) allyl disulfide [0.01%], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], allyl disulfide [0.016%] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) allyl disulfide [0.06%], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) allyl sulfide [0.12%], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], alverine citrate [500 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) amphotericin B [0.31 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) amsacrine [500 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) angelicin [62.5 uM], time [5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) angelicin [62.5 uM], radiation [unknown], time [5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) minimal complete -Arg [standard], time [overnight + 5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) aureobasidin A [0.02 ug/ml], time [5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) bathophenanthroline disulfonate [75 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) bathophenanthroline disulfonate [100 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) BCNU [62.5 uM], time [overnight + 20 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], BCS [100 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], BCS [200 uM] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [5 generations], benomyl [10 ug/ml] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 5 generations], benomyl [10 ug/ml] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) benzaldehyde [0.003%], time [5 generations] hom 4,742 Quantitative
Hillenmeyer ME~Giaever G, 2008 growth (relative abundance in pooled culture) time [overnight + 20 generations], berberine chloride [125 uM] hom 4,742 Quantitative

Curation history

Tested strains
May 3, 2016 Ready to load.
May 11, 2016 Loaded.

Data
May 3, 2016 Ready to load.
May 11, 2016 Loaded.